Ellison BS, Zanin MK, Boackle RJ
Get rewards Use your zooPoints to add free gifts from the Rewards Shop to your order
Primary patient-derived AML cells were isolated using Ficoll gradients and were cultured in IMDM (Thermo Fisher Scientific, USA) supplemented with 15% BIT 9500 Serum Substitute (STEMCELL Technologies, Canada), 100 ng/ml stem cell factor (SCF) (ImmunoTools, Germany), 50 ng/ml FLT3L (ImmunoTools, Germany), 20 ng/ml interleukin-3 (IL-3) (ImmunoTools, Germany), 20 ng/ml G-CSF (ImmunoTools, Germany), 0.1 mM 2-mercaptoethanol (Gibco, USA), 50 g/ml gentamicin (Thermo Fisher Scientific, USA), 10 g/ml ciproflaxin (Thermo Fisher Scientific, USA), 500 nM SR1 (APExBIO, USA) and 1 M UM279 (APExBIO, USA) at 37 C, 5% CO 2 , and 95% humidity
Although ROS can be generated at several sites of the ETC ( Figure 2
The nuclei were counterstained with DAPI and covered with ProLong Gold antifade reagent with DAPI (Life Technologies Corporation, OR, United States)
The glycolytic pathway of Saccharomyces cerevisiae was considered to be the intracellular ATP regeneration system for GSH synthesis [13]
